Hi - there are a couple of instruments that automate ISH and RISH. Our lab was a beta site for BioCare's Oncore and we loved it. It has an open platform and is very user friendly. The Oncore is a very compact instrument and the maintenance was easy and minimal. All of the techs here liked it. Check it out.
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 6 Date: Wed, 25 Jun 2014 08:38:29 -0400 From: Emily Brown <talulahg...@gmail.com> Subject: Re: [Histonet] ISH preparation To: jennifer.john...@genzyme.com That may be what you have to do, as the first day of ISH needs to be RNase free! When I do them, it is 10 slides at a time and we have dishes and a lab bench set aside for RNase free procedures only. Obviously this is not feasible in all workplaces! I would just think about it logically. Use gloves when you can, always. Can you keep the hybridizing part RNase free? I don't know what machines you use for this--we do process the slides manually in glass dishes and then hybridize in a slide container and oven kept for RNase free purposes. After hybridization, you don't really need to be too careful. Now I'm wondering if there's some sort of magical machine that does ISH! Emily ************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet