Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. I was wondering if anyone else has come across this, and if they have fixed it?
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