Beth,
We are doing exactly that same thing right now. This is changing a
complete detection system, so we need a full validation (10 pos + 10
negs for all non-prognostic markers). To avoid running 20 slides on
every antibody on our menu ($$$$), we have chosen to use a 3-prong method:
1. we bought appropriate TMAs from reputable vendors (such as
Pantomics, Inc) which will provide more than 10 Pos +10 neg for most cases.
2. we made a universal "negative" control with 12 different tissues
from our collection (provides tissues processed by us)
3. we run 1(or 2) of our control slides (bonus: also validates the
control at the same time)
This way we only run three slides for each validation to get the
required number of tissues - big difference in workload and cost!
Beth Cox, HTL/SCT(ASCP)QIHC
Pathology Solutions, Inc
__________________________________________________________________________
Message: 1
Date: Wed, 9 Jul 2014 12:16:55 -0500
From: Beth Brinegar<bbrinegar...@gmail.com>
Subject: [Histonet] Ventana ultraview users
To:"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
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<CAFYmSULScGQdaSdrU0dBz=55yaq-qlwsm_z944a1hdeniw7...@mail.gmail.com>
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Hello all,
Our lab is getting ready to switch from using the iview DAB to the
ultraview - any tips for validation? How many cases did you run for each
antibody?
Thanks!
Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403
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