I test our shower and eye wash weekly. We had a consulting company prepare us 
for an OSHA inspection and we were told then the testing needed to be done 
weekly for both. The temperate, flow, and clarity aren't necessarily tested 
just function. I use a 35 gallon trash bin to collect the water from the 
shower. I only run the shower enough to flush about 5 gallons out. 
Hope this helps! 

Alicia Lange HTL (ASCP)

> On Jul 11, 2014, at 10:00 AM, [email protected] wrote:
> 
> Send Histonet mailing list submissions to
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> Today's Topics:
> 
>   1. RE: safety shower (Amber McKenzie)
>   2. Job Opportunity (Baldwin, Kathy)
>   3. RE: Cytokeratin AE1/AE3 (Cartun, Richard)
>   4. formalin/xylene monitoring (Ronda Mire)
>   5. RE: formalin/xylene monitoring (Vincent Rivera)
>   6. Re: formalin/xylene monitoring (Ronda Mire)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 11 Jul 2014 14:36:14 +0000
> From: Amber McKenzie <[email protected]>
> Subject: [Histonet] RE: safety shower
> To: John Shelley <[email protected]>, Nancy Schmitt
>    <[email protected]>, "[email protected]"
>    <[email protected]>
> Message-ID: <[email protected]>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> What should the water volume be? Temperature and clarity?  How do you test 
> these things?
> 
> -----Original Message-----
> From: [email protected] 
> [mailto:[email protected]] On Behalf Of John Shelley
> Sent: Wednesday, July 09, 2014 12:39 PM
> To: Nancy Schmitt; [email protected]
> Subject: [Histonet] RE: safety shower
> 
> Our safety group here had built a neat little contraption using flexible PVC 
> piping and making it circular, slightly larger than the shower head and 
> placed a shower curtain and rings on it. When they pull the lever and use 
> their system the water falls into a garbage can for collection. They come 
> around once a month to check it out.
> 
> Kind Regards!
>  
> John J Shelley
> Research Specialist, Histology Core Facility
> 
> -----Original Message-----
> From: [email protected] 
> [mailto:[email protected]] On Behalf Of Nancy Schmitt
> Sent: Wednesday, July 09, 2014 1:29 PM
> To: [email protected]
> Subject: [Histonet] safety shower
> 
> How often are you testing your safety shower ?  Are you always checking for 
> water volume,  temperature and clarity?
> 
> I appreciate your thoughts.,
> 
> Nancy Schmitt MLT, HT(ASCP)
> Dubuque, IA
> 
> 
> 
> NOTICE: This email may contain legally privileged information. The 
> information is for the use of only the intended recipient(s) even if 
> addressed incorrectly. If you are not the intended recipient, please notify 
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> 
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> 
> 
> ------------------------------
> 
> Message: 2
> Date: Fri, 11 Jul 2014 14:49:35 +0000
> From: "Baldwin, Kathy" <[email protected]>
> Subject: [Histonet] Job Opportunity
> To: "'[email protected]'"
>    <[email protected]>
> Message-ID: <[email protected]>
> Content-Type: text/plain; charset="us-ascii"
> 
> Histology Tech position: Full time Monday thru Friday.  Jasper, Indiana 
> 47546Jasper is a regional center in southwestern Indiana, noted for its 
> German Catholic ancestral roots Jasper has often been called the "Wood 
> Capital of the World", boasting a large number of furniture companies, 
> including Kimball 
> International<http://en.wikipedia.org/wiki/Kimball_International> and 
> Masterbrand Cabinets<http://en.wikipedia.org/wiki/Fortune_Brands>. Jasper is 
> also home to the Southern Indiana Education Service Center (SIEC), Jasper 
> Engines & Transmissions (largest remanufacturer in the market), and to a 
> satellite campus of Vincennes 
> University<http://en.wikipedia.org/wiki/Vincennes_University>.
> 
> JOB SUMMARY:
> 
> Responsible for embedding, sectioning and cover slipping paraffin blocks and 
> staining tissues to provide the pathologist with accurately prepared 
> specimens for review and diagnosis. The employee works with specimens from 
> patients of all ages.  The employee must reflect mission statement and 
> philosophy of Memorial Hospital and Health Care Center in daily work habits 
> and contacts.
> QUALIFICATIONS:
> 
> Education:       High school diploma or equivalent is required.  Associate 
> degree preferred.  HT (ASCP) certification is preferred. Three years' 
> experience as histology assist is acceptable.
> 
> Training:          Six months on the job training required.
> 
> Experience:      Previous histology experience is preferred.  New graduates 
> of histology school will be considered.
> 
> S Kathy Baldwin
> Histology/Cytology Supervisor
> PH. 812-996-0210, Fax 812-996-0232
> [email protected]<mailto:[email protected]>
> 
> "Christ's healing mission of compassion empowers us to be for others through 
> Quality and Excellence"
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Fri, 11 Jul 2014 16:08:56 +0000
> From: "Cartun, Richard" <[email protected]>
> Subject: [Histonet] RE: Cytokeratin AE1/AE3
> To: "Rathborne, Toni" <[email protected]>, "Burnett, Brandy"
>    <[email protected]>, "'[email protected]'"
>    <[email protected]>
> Message-ID:
>    <9215bd4b0ba1b44d962a71c758b68d2e355b5...@hhcexchmb05.hhcsystem.org>
> Content-Type: text/plain; charset="us-ascii"
> 
> Reference:  Weaver DL, et al.:  Effect of occult metastases on survival in 
> node-negative breast cancer.  NEJM 2011;364:412-421.  A study funded by the 
> National Cancer Institute (Clinical Trials).
> 
> Conclusion - "These data do not indicate a clinical benefit of additional 
> evaluation, including immunohistochemical analysis, of initially negative 
> sentinel nodes in patients with breast cancer.
> 
> Also, from an NSABP study:  Role of IHC in invasive lobular carcinoma - 
> Although routine IHC staining with cytokeratin is not indicated for most 
> breast cancers, it can be helpful for examination of the sentinel nodes in 
> patients with invasive lobular carcinoma since the morphology of lobular 
> cancer can be difficult to detect on H&E of axillary lymph nodes [91,92]. In 
> general, IHC should be used to definitively diagnose an area that is 
> suspicious for, but not diagnostic of, lymph node metastases on H&E rather 
> than as a routine method of evaluating nodes in cases of invasive lobular 
> cancer.
> 
> I believe most major cancer centers have stopped doing cytokeratin IHC on 
> sentinel nodes for most breast cancer specimens.  I know that we have.
> 
> Richard
> 
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596 Office
> (860) 545-2204 Fax
> [email protected]
> 
> 
> -----Original Message-----
> From: Rathborne, Toni [mailto:[email protected]] 
> Sent: Thursday, July 10, 2014 4:49 PM
> To: Cartun, Richard; Burnett, Brandy; '[email protected]'
> Subject: RE: Cytokeratin AE1/AE3
> 
> Would you share the source for the  sentinel lymph node recommendation 
> mentioned? Although I just did a quick search, I didn't see anything on the 
> CAP website.
> 
> -----Original Message-----
> From: [email protected] 
> [mailto:[email protected]] On Behalf Of Cartun, 
> Richard
> Sent: Thursday, July 10, 2014 4:08 PM
> To: Burnett, Brandy; '[email protected]'
> Subject: [Histonet] RE: Cytokeratin AE1/AE3
> 
> "Extra-follicular reticulm cells" contain cytokeratin and will label with 
> antibodies to low molecular weight cytokeratin.  Could that be what you are 
> seeing?
> 
> Reference:  Linden MD and Zarbo RJ:  Cytokeratin immunostaining patterns of 
> benign, reactive lymph nodes:  applications for the evaluation of sentinel 
> lymph node specimen.  Appl Immunohistochem Mol Morphol 2001;9(4):297-301.
> 
> On another note,  the recommendation now is to not do CK IHC on sentinel 
> lymph nodes (except for rare cases).  Lymph node involvement should be based 
> on H&E; not IHC.
> 
> Richard
> 
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs Assistant Director, Anatomic 
> Pathology Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596 Office
> (860) 545-2204 Fax
> [email protected]
> 
> 
> -----Original Message-----
> From: [email protected] 
> [mailto:[email protected]] On Behalf Of Burnett, 
> Brandy
> Sent: Thursday, July 10, 2014 12:08 PM
> To: '[email protected]'
> Subject: [Histonet] Cytokeratin AE1/AE3
> 
> Hi Histonetters,
> 
> I just came across a thread regarding issues with Cytokeratin AE1/AE3 from 
> DAKO. I am having the same issues with our New protocol using HIER on the 
> Link-48 in which sentinel lymph nodes from breast cases are staining 
> non-specifically "in-between the cells".
> We never had this issue before on our old Autostainer plus, however, we were 
> using only enzyme pre-treatment on it before staining.
> I was wondering if anyone else has come across this, and if they have fixed 
> it?
> 
> Thank you,
> 
> Brandy Burnett, HTL
> Cape Cod Hospital
> 
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> 
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> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 11 Jul 2014 12:41:04 -0400
> From: Ronda Mire <[email protected]>
> Subject: [Histonet] formalin/xylene monitoring
> To: "<[email protected]>"
>    <[email protected]>
> Message-ID: <[email protected]>
> Content-Type: text/plain; charset=us-ascii
> 
> 
> Hello Netters
> 
> I just received my CAP self inspection checklist and I do not see the 
> question regarding Xylene/Formaldehyde monitoring.  Has this checklist item 
> been removed?  Is monitoring no longer necessary?  What gives?  I have 
> checked the CAP website but haven't found anything.
> 
> Ronda Mire
> CVPath Institute
> Gaithersburg, MD
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Fri, 11 Jul 2014 16:49:10 +0000
> From: Vincent Rivera <[email protected]>
> Subject: RE: [Histonet] formalin/xylene monitoring
> To: 'Ronda Mire' <[email protected]>,
>    "<[email protected]>"
>    <[email protected]>
> Message-ID:
>    <3d4a471b82e7a44c87f6839732320d9f013f32c...@vspdms-itexmb02.dms.com>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi Ronda,
> 
> It is located in the Anatomic Pathology Checklist safety section, I believe.
> 
> Vincent Rivera, HT (ASCP), QIHC, QLS
> Histopathology Supervisor
> West Dermatology Pathology Laboratory
> [email protected]
> 714-924-7240 (Lab)
> 714-390-0906 (Cell)
> 
> -----Original Message-----
> From: [email protected] 
> [mailto:[email protected]] On Behalf Of Ronda Mire
> Sent: Friday, July 11, 2014 9:41 AM
> To: <[email protected]>
> Subject: [Histonet] formalin/xylene monitoring
> 
> 
> Hello Netters
> 
> I just received my CAP self inspection checklist and I do not see the 
> question regarding Xylene/Formaldehyde monitoring.  Has this checklist item 
> been removed?  Is monitoring no longer necessary?  What gives?  I have 
> checked the CAP website but haven't found anything.
> 
> Ronda Mire
> CVPath Institute
> Gaithersburg, MD
> _______________________________________________
> Histonet mailing list
> [email protected]
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> ------------------------------
> 
> Message: 6
> Date: Fri, 11 Jul 2014 12:57:09 -0400
> From: Ronda Mire <[email protected]>
> Subject: Re: [Histonet] formalin/xylene monitoring
> To: Vincent Rivera <[email protected]>
> Cc: "<[email protected]>"
>    <[email protected]>
> Message-ID: <[email protected]>
> Content-Type: text/plain; charset=us-ascii
> 
> Found it,  Thanks !!!
>> On Jul 11, 2014, at 12:49 PM, Vincent Rivera <[email protected]> wrote:
>> 
>> Hi Ronda,
>> 
>> It is located in the Anatomic Pathology Checklist safety section, I believe.
>> 
>> Vincent Rivera, HT (ASCP), QIHC, QLS
>> Histopathology Supervisor
>> West Dermatology Pathology Laboratory
>> [email protected]
>> 714-924-7240 (Lab)
>> 714-390-0906 (Cell)
>> 
>> -----Original Message-----
>> From: [email protected] 
>> [mailto:[email protected]] On Behalf Of Ronda Mire
>> Sent: Friday, July 11, 2014 9:41 AM
>> To: <[email protected]>
>> Subject: [Histonet] formalin/xylene monitoring
>> 
>> 
>> Hello Netters
>> 
>> I just received my CAP self inspection checklist and I do not see the 
>> question regarding Xylene/Formaldehyde monitoring.  Has this checklist item 
>> been removed?  Is monitoring no longer necessary?  What gives?  I have 
>> checked the CAP website but haven't found anything.
>> 
>> Ronda Mire
>> CVPath Institute
>> Gaithersburg, MD
>> _______________________________________________
>> Histonet mailing list
>> [email protected]
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> ------------------------------
> 
> _______________________________________________
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> [email protected]
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> End of Histonet Digest, Vol 128, Issue 10
> *****************************************

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