Good Morning,

I have avoided for the last few years doing IHC with mouse on mouse antibodies, 
but have been requested to do so at this time. I have two antibodies, HDAC1 and 
Nitrotyrosine from Santa Cruz that I am working up, however the staining 
pattern in my tissue does not seem to be revealing the accurate staining 
pattern. I was hoping someone in the research community has worked with these 
antibodies, even if from other vendors and would be willing to discuss further 
with me. Thanks!!!

Kind Regards!

John J Shelley
Research Specialist, Histology Core Facility
Sanford-Burnham Medical Research Institute at Lake Nona

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