Hi All,
I was wondering what stain protocols are being used for Calponin to minimize cross-reactivity with myofibroblasts? We are using a Dako concentrate antibody. I recently re-optimized the stain using a 1:100 dilution and changed the pre-treatment from low pH HIER, to Protease only. This helped a lot with cross-reactivity with myofibroblasts that we were seeing, however we are still seeing some non-specific staining from time to time. Any information would be greatly appreciated! Thanks, Brandy Burnett Cape Cod Hospital ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
