Hello Amber, That depends on your protocol. Which protocol are you using and what does your protocol say to use the schiffs at? Consider that schiffs is usually stored at 4C in brown or opaque bottles. This suggests it is light sensitive. Our protocol say to use the schiffs at 37C for 15 minutes but I have used it at 22C for 30 minutes and at 60C for 10 minutes with consistent results. We have found that using it at 60C reduces it's shelf life considerably and do not suggest it unless you are familiar with doing so.
Good luck Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie <amber.mcken...@gastrodocs.net> wrote: > > When staining PAS's, does the Schiff need to be room temp or does it matter? > Just curious about when to add it to our Sakura stainer. Should we put the > schiff's on at the beginning of our shift so it's room temp when we need it > or when should add it when we add PAS slides to it that need to be stained? > Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
