Many labs have turned to tissue microarrays (TMAs) for validating antibodies for IHC testing as a matter of convenience and lower cost. Ideally, the tissues used to make the TMA(s) should be fixed in the formalin that is used in your laboratory, as well as processed in your Histology laboratory under identical conditions as those used for patient specimens.
Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Sunday, August 03, 2014 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC validation: Does anyone have experience validating antibodies using tissue microarrays? Is this a preferred method? I was thinking of punching different tissues/cases, then embedding them in one block, and then validating the antibody. Please advise. Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
