Teri~ Thanks so much for the very useful feedback... We have only encountered this problem recently and these suggestions will be very useful!
~Brandy ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Terri Braud [tbr...@holyredeemer.com] Sent: Friday, August 15, 2014 1:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue falling off Hi - Just a couple of suggestions - I've seen this periodically crop up during the years. There are a couple of things to consider: Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol. Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off. Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths. Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack. Hope this gives you a few ideas Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 7. tissue falling off slides (Burnett, Brandy) From: "Burnett, Brandy" <bburn...@capecodhealth.org> Subject: [Histonet] tissue falling off slides Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated. Thanks Again, Brandy Burnett, HTL --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
