Hello colleagues I am using this machine since long time. P/V mostly is not the cause of tissue drying, Check the wax baths temperature if it's not over 2 degrees from the paraffin wax melting point. Make sure the temperature in Xylene steps is not over 37 C degree. Check the purity of the ethanol, specially the absolute ethanol.
Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories *Sent from my cell phoner* -------- الرسالة الأصلية -------- من: Ian R Bernard <ibern...@uab.edu> التاريخ: ٢٠١٤/٠٨/٢٩ ٦:٥٢ ص (GMT+04:00) إلى: "Algeo, Lacie A" <lacie.al...@providence.org>,histonet@lists.utsouthwestern.edu الموضوع: [Histonet] RE: processing biopsies on VIP Check your protocol. Heat or temp is a factor and length of time in alcohol. We have P/V for the 10 % NBF and paraffin infiltration. IB -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Thursday, August 28, 2014 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing biopsies on VIP Hi there, Does anyone use P/V on their biopsy runs on the Tissue-Tek VIP 5? We're having really dry biopsies, and I think this could be why.... Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.al...@providence.org<mailto:lacie.al...@providence.org> This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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