Hi Histonetters
Has anyone else had difficulty getting this clone to work? cd4 1F6 from leica
microsystems (formerly novocastra).
I've tried just about every trick in my book and still no staining.
Particularly i made sure i did the peroxidase block prior to HIER (i even tried
using it after the primary) and have tried low, high and middle pH buffers with
antibody concentrations from 1/10-1/40 for 60 and 90 mins and use envision plus
detection with DAB plus. I've had two batches of this over the last ten years
and neither has worked.
Anyone else with the same experience ?
regards
steve weston
lab manager
Breathe-Well CRE
UTAS-SOM
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