Hi Jim, You mention that the OCT seems a little softer. Could it be that the sections are thicker than you usually produce. Check the Frozen section H&E to check this. If sections are thicker they will tend to detach from the slides.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, 3 September 2014 12:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sections for Immunoflouresence Lately we are experiencing an issue where the tissue sections are coming off of the slide during the staining process. We have always used positive charged slides and have not experienced this problem on a routine case. We do not fix the slides but allow them to air dry for Immunology. Some have suggested a quick fixation in acetone or 95% ETOH. This doesn't seem to correct the problem and we don't find the IF stains work as well. We have tried a different batch of slides and are looking into a different kind of coating for the slides. Our regular frozen sections stay on the slides. We are using OCT. The only other thing I have noticed is that maybe it's my imagination but the OCT in the block surrounding the kidney biopsy seems a little softer than normal. I did try a different mounting medium and maybe the softness was my imagination. Has anyone else experienced this problem and if so how did you correct it? The lab is air conditioned so I don't think the humidity is the main factor. The Immunology staff changed all of their reagents so we have reduced that variable hopefully. We are using Leica positive charged slides. (I have found that the Apex control box slides from Leica are a little better than the regular Apex slides. Any ideas? Jim ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
