I used to have a triangle glass knife holder insert for my Leica microtome or I would use the tungsten carbide knives. It depends on what you are cutting. if it is calcified bone the glass knives scratch too much and they are only 1/2 inch wide so you have to cut smaller soft tissues with them.
Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net From: abri...@brightinstruments.com Date: Fri, 12 Sep 2014 17:32:47 +0100 To: gkey...@uwhealth.org Subject: Re: [Histonet] Blades for cutting resin on a microtome CC: histonet@lists.utsouthwestern.edu; veronique.bar...@gmail.com; exp...@brightinstruments.com Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes, KR,,,,,Alan Bright Sent from my iPhone > On 12 Sep 2014, at 15:49, "Keyser Gerald T" <gkey...@uwhealth.org> wrote: > > I've only cut resin with a glass or diamond knife in an ultramicrotome. If > you are attempting to do it in a regular microtome, you would need a special > blade holder. I don't know if any microtome manufactures make glass knife > holders. > > You make the glass blades yourself using special glass. Here is a link to the > glass strips: > http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=en®ion=US > > Here is a cheap jig and diamond glass cutters it make the knifes: > https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx > > I've never made glass knives by hand using a hand held diamond cutter and > jigs. I imagine that it would take practice. > > I've only used a specialized maker: > https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx > > You paint a bit of nail polish underneath the glass edge and put a bit of > distilled water on the edge. You then section the block floating the sections > on the water. Use an eyelash manipulator to pick up the 5um thick sections > and place on a bubble of water on the slide. Evaporate the water droplet on > the slide. If you've done it right, the sections won't look like origami. If > it does, then practice until it doesn't. > > Gerry > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique > Barrès > Sent: Friday, September 12, 2014 9:33 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Blades for cutting resin on a microtome > > Happy Friday Histonetters! > > I am working on a histology platform in a research center and someone came to > me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I > never cut anything else than paraffin, so I was wondering if some of you had > advices for me? > > They never did it neither and took their protocol in a paper where it was > said that we should use disposable glass knife instead of standard metal > blades. Are any of you ever used those knife? Where do you buy them? > We have an old Leica RM2125. > > Thanks for your advices! > > Véronique > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
