We have used a hardmount Thermo "Immumount" Cat# 9990402. It is the only hardmount we tested that gave good focus using the NanoZoomer scanner. No need to seal with nail polish, saving a lot of time. We just re-scanned slides that were stained 6 months ago and still had great fluorescence. We use life Technologies "Alexa Fluor" secondary antibodies.
James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Segovia, Claudia Sent: Monday, September 15, 2014 8:36 AM To: Christina Kreutzer Cc: <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] mounting media for immunofluorescence Hi Christina, We use DPX. It has worked very well for us. I am not sure about the cost though, but anything bought in kits can get really pricy. DPX comes in 500 ml. Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csego...@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csego...@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us. > On Sep 15, 2014, at 11:21 AM, "Christina Kreutzer" > <christina.kreutze...@gmail.com> wrote: > > Hello folks, > I am wondering which mounting medium für IF you are using in your > daily routine. > We are using Prolong antifade gold, but it is really really really > expensive and I was wondering if there is a good alternative. I just > ordered a sample of the Dako Fluorescence Mounting Medium so we can > try that , but we are a little bit concerned about how long lasting > the fluorescence signal would be. > > Well, what are your experiences so far. > > All the best > Christina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
