On fatty tissue, especially cheeks. After we mount on a slide we spray the tissue with liquid nitrogen and let sit to freeze before embedding for a couple of minutes. Than after embedding spray the chuck again with liquid nitrogen. On really fatty stuff you may have to freeze before cutting each time. Time consuming yes. But I find freezing and let sitting I don't fight with it as much when cutting. Anne
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of michele carr Sent: Tuesday, September 23, 2014 9:00 PM To: [email protected] Subject: [Histonet] Mohs Hello I perform mohs cutting a few days a week and I noticed that on fatty skin tissue I don't get the interior of the skin section. I only seem to get the epidermis. Is there a trick to getting the fatty tissue too. I tried bumping up the microns but that only helped a bit. Thanks for your responses. Michele Carr _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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