With the 100% ETOH, Histoclear, and paraffin, you will need two changes as has already been mentioned. Dense, thick or fatty tissues require more time. Given these are small animal tissues you may be able to cut your time in each station in half. You also may think about using isopropanol rather than the more expensive ethanol. So, add a change of each as above and try processing in each one 30 minutes and see how that works for you. This makes this about a 5 hour process which may be more doable. However, you may find that without pressure/vacuum in the paraffin you may need one hour in each change for good infiltration.
Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
