Sadly we don't have the capacity to do frozen sections :( Christy
-----Original Message----- From: Diane G. Miller [mailto:dgmil...@coho.net] Sent: October 23, 2014 11:56 AM To: Thompson, Christy Subject: Re: [Histonet] fish eggs: processing and fixation Have you tried doing frozen sections instead of processing them in paraffin. We use to do frozen sections on frog eggs that were fixed and it worked picking them up on charged slides. Diane -----Original Message----- From: Thompson, Christy Sent: Thursday, October 23, 2014 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish eggs: processing and fixation Hi all, I'm currently working on some unfertilized fish eggs. They were fixed in 10% NBF and processed in a vacuum processor with paraffin. The eggs are extremely hard to section and most of the yolk goes AWOL on the way to the water bath. I did do a search here for some help and a few things were posted but one post in particular found some answers but they were not listed in his reply and I think the post was pretty old (but no date on it). Anyone out there have any success with sectioning fish eggs? Thanks in advance! Christy Thompson A/Histology Lab Leader - Aquatic Animal Health Pacific Biological Station Fisheries and Oceans Canada Nanaimo, BC 250-756-7060 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet