We bake ours @ 75-80 degrees for 15 minutes in an offline oven, prior to automated staining. Just a habit I picked up years ago, but I always lean the slide racks diagonally against the side of the oven when they're baking, rather than having them sit entirely upright. They seem to drain any residual water more completely that way. We've used Fisher's positive charged slides for years and haven't really had a problem with tissue loss on H&E. I think your baking temp and time may be a little low and short, respectively.
Good luck. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, November 06, 2014 8:36 AM To: Pardue, Judith Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue falling off positive slides Make sure yr sections r airdryed completely before u bake thm Sent from my iPhone > On Nov 6, 2014, at 10:34 AM, "Pardue, Judith" <judith_par...@memorial.org> > wrote: > > WE are having trouble with tissue coming off our h&e slides. Our heater is > set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue > positive slides. > > Judith Pardue > CHI Memorial > Chatt. Tn. 37343 > judith_par...@memorial.org > > This electronic mail and any attached documents are intended solely for the > named addressee(s) and contain confidential information. If you are not an > addressee, or responsible for delivering this email to an addressee, you have > received this email in error and are notified that reading, copying, or > disclosing this email is prohibited. If you received this email in error, > immediately reply to the sender and delete the message completely from your > computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet