agree Biocares Warp Red is very strong but still has to avoid alcohol. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net
From: l...@premierlab.com To: yves.herem...@vub.ac.be; histonet@lists.utsouthwestern.edu Date: Thu, 6 Nov 2014 13:43:09 -0700 Subject: RE: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red... CC: Yves Try Biocares Warp Red, you will still need to air dry the slides prior to coverslipping do not place them in alcohol, air dry, place in xylene and then mount. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Thursday, November 06, 2014 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red... Dear Histonetters, I am trying to combine a peroxidase-DAB staining (brown) with an alkaline phosphatase-staining for detection of two proteins that do not overlap. I have already tried Dako’s Permanent Red and Fuchsin+ and Vector’s Vector Red as AP substrates but I am not getting a clear red color, rather something brownish which is not very different in color from the DAB reaction product. Is there anything I can do to obtain a clearer red color ? Yves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
