Hi Claudia, We use Dako anti-Human Tau (rabbit polyclonal) at a concentration of 1:8000. We do not use a pretreatment (HIER or protease). Staining is done on a Ventana stainer.
Good luck, Willem ________________________________________ Van: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] namens Segovia, Claudia [csego...@nsalabs.com] Verzonden: donderdag 6 november 2014 19:23 Aan: <histonet@lists.utsouthwestern.edu> Onderwerp: [Histonet] Endogenous Tau (normal Tau) Hi there, I am writing a poster on different Tau epitopes in human brain free floating sections. I have been unable to get any normal Tau and I wonder what could be the problem. Does anybody know what could be? Also, does anyone have a protocol that can share with me? Thank you! Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csego...@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csego...@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
