Well if the specimens are well fixed in formalin, then bring back the colour with alcohol and you can the store them in a non-formalin solution such as dilute honey or high salt solution:
Henwood, A., (2002) "Color preservation in pathology museum specimens" Biotechnic & Histochem 77(4):230. Özkan, N., Salva, E., Cakalagaoglu, F., & Tüzüner, B. (2012). Honey as a substitute for formalin?. Biotechnic & Histochemistry, 87(2), 148-153. Al-Maaini R, Bryant P (2006) The effectiveness of honey as a substitute for formalin in the histological fixation of tissue. J. Histotechnol. 29: 173–176. Oliveira, F. S. (2014). Assessing the effectiveness of 30% sodium chloride aqueous solution for the preservation of fixed anatomical specimens: a 5‐year follow‐up study. J. Anat. (2014) 225, pp118-121 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Friday, 21 November 2014 8:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. *********************************************************************************
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