Joanna, I agree with Tim... We also see this at times if the tissue has freezing artifact. Gomori Trichrome is very unforgiving :)
Sarah E. Lewis HT, ASCP The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -----Original Message----- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Thursday, December 11, 2014 11:19 AM To: 'Joanna'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gomori Trichrome Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v2/url?u=http-3A__mailto-3Ahistonet-2Dbounces-40lists.utsouthwestern.edu&d=AAIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=7bt8tbqem5cRuX3hC3eOeQ&m=0vo4hfofYzOL04nyQnusz7sZCdl6SlMBYQxPhvKuLTo&s=9bOOBwtwMikwEx-4zwlWv08DLvU_t-ipNcKOpMi5GfA&e= ] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AAIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=7bt8tbqem5cRuX3hC3eOeQ&m=0vo4hfofYzOL04nyQnusz7sZCdl6SlMBYQxPhvKuLTo&s=j_zGkNIiR0dF7a9QsR9pyFxqkZn_Q4JNpgF8pigo2UU&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
