We would like to use Toluidine Blue for staining frozen sections of kidney biopsy. We finally are able to get the stain dark enough, but we can't get rid of the background caused by the OCT. We've washed with water, alcohols, and PBS with no success. Is anyone out there using Toluidine Blue for frozen staining? If so, what is your recipe and how do you get rid of the background? Thanks
Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC one...@wvuhealthcare.com<mailto:one...@wvuhealthcare.com> Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet