Hi, We've had really good results with pretreatment in 1% Uranyl Nitrate, as well as with 1% Uranyl Acetate in Acetate Buffer--both at room temperature, and at 60 degrees C for 10 minutes. We've also tried zinc formalin, as well as "Spirochete Sensitizer"--a commercially available product, but we've only obtained extremely faint staining of spirochetes with the latter two. Our sections are cut at 4 µm. Thanks,
Brian -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:j...@cdc.gov] Sent: Wednesday, December 24, 2014 5:13 AM To: Cooper, Brian; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Warthin Starry on Dako Artisan What protocol with uranyl nitrate do you use? Dako told us to use zinc formalin as a sensitizer but it doesn't seem to make much of a difference. Thanks! -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 23, 2014 11:18 AM To: Cooper, Brian; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry on Dako Artisan Try a 0.10% solution of phosphotungstic acid.René J. On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" <bcoo...@chla.usc.edu> wrote: Happy Holidays Histonetters! Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate? With offline uranyl nitrate pretreatment, spirochetes stain brilliantly! But everything else we've tried leaves them invisible . . . Any guidance would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ---------------------------------------------------------------------
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