I can help with the old fashioned advice:
- 1 scant teaspoon simple syrup - 2 dashes Angostura Bitters, plus more to taste - 1 half dollar–sized slice orange peel, including pith - 2 ounces good-quality rye or bourbon - 1 maraschino cherry As for the Histology, is there any reason you cannot mount the sections onto glass slides? When I was working at Genentech they were cutting frozen sections through whole rabbits and mounting the sections on (giant) glass slides. I think that rolling the tissue up, inserting it, and then removing it from a glass tube would destroy the tissue. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia <mbmph...@gmail.com> wrote: > First, the very best of holidays to everyone. > > Now for the histology part. Our lab's focus is on the early stages of > Alzheimer's Disease in the Brainstem > using celloidin processing & embedding for IHC staining. This year, our > lab will be receiving 6 post-mortem > whole human brains (1 every other month). After fixation, processing & > celloidin embedding, the whole brain > will be serially cut at 100um thick. Each brain section will be 5 inches > x 4.5 inches in size. > > I will given 250 of these whole brain sections to stain for tau > IHC...that's 1500 whole brain sections/year!!! > 1) Does anyone have experience doing manual IHC staining of large > free-floating brain sections? > 2) What type of staining tools, dishes or other essential equipment can > anyone recommend? > 3) What's the most efficient way to stain 250 sections for batch IHC > staining - such as transferring batch > sections (maybe 5-10) from reagent to reagent? > 4) What type of batch apparatus to use? > > As for the antibody & ABC steps, I was thinking of placing each section > inside a large glass cigar tube > (yep, people use large glass tubes with fitted cap to store cigars), with > 5ml of antibody or ABC reagent & gently agitate on > a shaker/rotator at room temp during the incubation. Does anyone have > ideas on this? > > Please, any ideas, suggestions or recommendation anyone can provide will > be most greatly appreciated. > > Best regards > Maria Mejia > UCSF > Department of Neurology > San Francisco, CA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet