Hello, I believe that the products for the CryoJane tape transfer are still available from Fisher.
Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St Room 310 Basement| Bond St Annex Building Baltimore, MD | 21231 410-614-1660 http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, February 05, 2015 1:08 PM To: Orla M Gallagher; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cryosectioning undecalcified bone Orla There is an article in the Journal of Histotechnology from a while ago from John Tarpley that addressed methods for this. I have a pdf of it and I will send in another e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher Sent: Thursday, February 05, 2015 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryosectioning undecalcified bone Dear Histonetters, We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections - marrow without bone or bone without marrow on the slide, in spite of various freezing and prep. protocols used by my colleague. I suspect the old cryostat and blade weren't helping either. Have you any recommendations on the best cryostat to use to do this? We'd like to also use the cryostat for standard soft tissue sectioning. I've seen Leica mentioned in a few papers relating to CryoJane. There appears also to be a tape transfer method from Kawamoto's Section-Lab Co. Ltd. (i...@section-lab.jp) Does anyone use this method or know whether the consumables are still available for purchase, as the website seems dormant? Thanks for any advice, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/humanmetabolism/greenimpact <http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording> http://www.sheffield.ac.uk/visitors/mapsandtravel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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