Please give your processing schedule with times and reagents? It is hard to help without knowing how the tissue was handled from sacrifice to embedding. Pam Marcum
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Marshall Sent: Wednesday, February 18, 2015 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help with cutting mouse brain at 9-10 Microns ?Hello Histo folks I am starting a research project with mouse brain, I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all. I have soaked them in warm water and wonder in using a softener like conditioner would help. I am new to the animal tissue world and any advise would help. Thanks in advance. Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
