Hi Yak-Nam, Have you thought of using Picrosirius Red staining? We use it to assess changes in the collagen fibres. Under polarised light, the collagen fibres exhibit birefringence (red/orange or green depending on fibre size) and the birefringence is lost/ becomes fainter as the collagen becomes degraded. You can boil spare tissue samples for different lengths of time to act as control/reference blocks for comparison. Hope that helps.
Andrew Prior Histologist Tissue Regenix Group Heslington, York YO10 5NY E-mail: a.pr...@tissueregenix.com<mailto:a.pr...@tissueregenix.com> Website: www.tissueregenix.com<http://www.tissueregenix.com/> Message: 5 Date: Tue, 24 Feb 2015 07:30:19 -0800 From: Yak-Nam Wang <ynw...@u.washington.edu<mailto:ynw...@u.washington.edu>> Subject: Re: [Histonet] gelatin To: John Kiernan <jkier...@uwo.ca<mailto:jkier...@uwo.ca>> Cc: "histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>" <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Message-ID: <caokigw+65opj_z9dto84goeprz4qtvrfrtptxzmn56otmbe...@mail.gmail.com<mailto:caokigw+65opj_z9dto84goeprz4qtvrfrtptxzmn56otmbe...@mail.gmail.com>> Content-Type: text/plain; charset=ISO-8859-1 Thank you for your e-mail. Apologies for not explaining "treated tissue". We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. Thank you for your thoughts Yak-Nam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
