Dear Histonet, I am making a 20% PVA solution for cryo embedding murine bones, following John Tarpley's recipe found in his JOH article, March 2003. He mentions no problems in making it, but after 3 days on the stir plate, it still has not gone into solution. The PVA settles very quickly, even before I can pour it into a mold to freeze and test. Is there a trick to this that I need to know? Any advice would be so great. Thank you, Nancy Thomas
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