Hi Anna, Working in Aquaculture, we section fish tissues daily - typically Salmonids. What works for us is routinely process to paraffin, then surface/trim the block followed by surface decal (for at least an hour) using a hydrochloric acid based product (RDC, Cellpath UK.)
Whole eyes do not process well unless very small, therefore we lay the eye cornea side down and cut out a transverse section on either side of the optic nerve prior to processing - hope this makes sense! It can help sectioning if the skin is positioned in the block perpendicular, rather than horizontal, to the blade. Prior to sectioning, the blocks are chilled for a few minutes- skin is placed face down on the cold plate eyes are placed face-up on the cold plate with a drop of distilled water on the lens to aid sectioning. What species of fish are you working with? Some cause more problems than others. Hope this helps! Debbie Debbie Faichney, BSc Senior Technician Histology/Bacteriology Laboratories Institute of Aquaculture University of Stirling Stirling, FK9 4LA Scotland UK Tel: +44(0)1786 466592/466590 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Coffey, Anna (NIH/NCI) [C] Sent: 20 March 2015 16:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fish Skin Histology Hello Histonet, I am looking for advice on the best method to prepare fish skin and eyes for sectioning. If you have had experience with this in the past, I'd love to hear what worked for you. Thanks in advance! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.cof...@nih.gov 301-846-1730 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling has been ranked in the top 12 of UK universities for graduate employment*. 94% of our 2012 graduates were in work and/or further study within six months of graduation. *The Telegraph The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
