Our facility implemented the post fixing of IHC slides years ago only for tissues like breast, cell blocks, or anything that tends to fall off. We baked the slides in the oven for 30 minutes, and then post fixed them for an additional 30 min in formalin 'fumes'. It works great on breast resections specimens, and it doesn't interfere with staining. Thank you,
Cecilia M. Obregon Memorial Regional Hospital 3501 Johsnon Street Hollywood, FL 33021 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Barry Rittman [barryritt...@gmail.com] Sent: Saturday, March 21, 2015 2:20 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Post fixing IHC slides in formalin There are always discussions about fixation but I have never seen comments about using vapor fixation for post fixing or for fixing fresh frozen specimens. Vapor fixation is simple to use and does not require any solvent as it uses the tissue fluids in the case of fresh frozen sections or the solution remaining after IHC. Especially useful for formaldehyde, alcohol, acetone, osmium tetroxide etc. Barry On Sat, Mar 21, 2015 at 12:14 PM, Ann Specian <thisis...@aol.com> wrote: > Does anyone post fix their IHC slides in formalin in an effort to try to > reduce tissue loss? If so, does anyone have a protocol for this that they > have used and have seen good results? > > > If you have any other suggestions which can help to reduce tissue loss > during IHC staining, I would love to hear from you. > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet