I can have them ready tonight or tomorrow Cynthia Tily Hutchinson Rsch Asst IV Pathobiology 166 Greene Hall Coll of Vet Med Auburn Univ 36849 (334)844 7020
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, March 11, 2015 11:58 AM To: [email protected] Subject: Histonet Digest, Vol 136, Issue 13 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. anti-BRAF V600E (VE1) Ventana (Susan Foreman) 2. RE: RE: Old slides. (suetp918) 3. PRN (Kristy Castillo) 4. Re: anti-BRAF V600E (VE1) Ventana (Patrick Laurie) 5. Open histology positions: San Francisco (Cheryl) 6. Direct Hire Histotech South of Tucson, AZ-New Grads Welcome to Apply! (Melissa Owens) 7. Job opportunity (Hunter, Theresa) 8. TDP43, PTG poly ([email protected]) 9. RE: Re: Bird head stored in 70% alcohol and possible decalcification (Rui TAHARA) 10. BIG day today! (David Kemler) 11. PA (Mike) 12. Re: PA (Jennifer MacDonald) 13. Diff Quik troubleshooting (Nancy Schmitt) 14. THICK AND THIN SECTIONS ? (Klaus Dern) 15. RE: Old slides (Mayer,Toysha N) 16. 5-methylcytosine IHC in tissue (Mariela Chertoff) 17. RE: Masson Trichrome stain (Mayer,Toysha N) 18. RE: Old slides (Marcum, Pamela A) 19. RE: Histonet Digest, Vol 136, Issue 12 (Solis, Bryan) ---------------------------------------------------------------------- Message: 1 Date: Tue, 10 Mar 2015 13:15:05 -0400 From: "Susan Foreman" <[email protected]> Subject: [Histonet] anti-BRAF V600E (VE1) Ventana To: <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring Bioscience or Ventana? What dilution are you using? Are you utilizing amplification? Expensive ------------------------------ Message: 2 Date: Tue, 10 Mar 2015 13:31:58 -0400 From: suetp918 <[email protected]> Subject: RE: [Histonet] RE: Old slides. To: "Gowan,Christie C" <[email protected]>, 'Bernice Frederick' <[email protected]>, "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset=utf-8 So we actually cut the film around the section and mount to another slide and do pretty much what wascabove mentioned placing upside downband placing on paper towel. ??Actually works pretty good. TJUH Sue Paturzo Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Gowan,Christie C" <[email protected]> Date:03/09/2015 4:01 PM (GMT-05:00) To: 'Bernice Frederick' <[email protected]>, [email protected] Subject: [Histonet] RE: Old slides. Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: [email protected] Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 [email protected]<mailto:[email protected]> _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 10 Mar 2015 11:11:54 -0700 From: Kristy Castillo <[email protected]> Subject: [Histonet] PRN To: "'[email protected]'" <[email protected]> Message-ID: <DED6D994175B70449148C3DEFCF034958C6119CB00@exchange> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I am looking to fill a PRN Histotech position in the Phoenix area. Does anyone know anyone who is retired and looking for PRN Histology work? Please feel free to e-mail me at the following [email protected]<mailto:[email protected]> Thank you! ________________________________ This transmission may contain confidential information, some or all of which may be protected health information as defined by the federal Health Insurance Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is intended for the exclusive use of the individual or entity to whom it is addressed and may contain information that is proprietary, privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient (or an employee or agent responsible for delivering this transmission to the intended recipient), you are hereby notified that any disclosure, dissemination, distribution or copying of this information is strictly prohibited and may be subject to legal restriction or sanction. Please notify the sender by telephone to arrange the return or destruction of the information and all copies. ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 14:48:23 -0400 From: Patrick Laurie <[email protected]> Subject: Re: [Histonet] anti-BRAF V600E (VE1) Ventana To: Susan Foreman <[email protected]> Cc: "[email protected]" <[email protected]> Message-ID: <cakeyg-1hzdazkjrbyiqjaczx2gpn18tjgg_oqdi-0lyx+4e...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Spring Bioscience is part of Ventana, Spring had the original clone, and now so does Ventana. We use the spring bioscience concentrated antibody on the Bond III at 1:100 dilution, using the Bond Hi-pH ER2 retrieval for 20 minutes. I know that Ventana sells the RTU version. Remember that from Spring it is RUO, so it is up to you to do the appropriate LDT. The Ventana antibody is an IVD. It is also a pretty pricey antibody from either vendor. Let me know if you need any further help. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Mar 10, 2015 at 1:15 PM, Susan Foreman <[email protected]> wrote: > What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring > Bioscience or Ventana? What dilution are you using? Are you utilizing > amplification? Expensive > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Tue, 10 Mar 2015 12:37:23 -0700 From: Cheryl <[email protected]> Subject: [Histonet] Open histology positions: San Francisco To: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=us-ascii Happy Histology Day, everyone! I'm looking for techs for my new (to me) lab!! I'm working at CPMC Sutter in San Francisco and we need a couple of permanent hires for our team. First few days it looks like it's gonna be fun - rock'n'roll kinda place where quality and speed have equal bearing. Let me know what you're looking for and let's see if it's a match! Email resumes to me at the email, below. Please feel free to share and THANK YOU! Cheryl Kerry, HT(ASCP) [email protected] ------------------------------ Message: 6 Date: Tue, 10 Mar 2015 20:28:30 +0000 From: Melissa Owens <[email protected]> Subject: [Histonet] Direct Hire Histotech South of Tucson, AZ-New Grads Welcome to Apply! To: "[email protected]" <[email protected]> Message-ID: <d124cc2a.76634%[email protected]> Content-Type: text/plain; charset="us-ascii" Hello Histonet, I am in search of a histotech for a permanent position about an hour south of Tucson, AZ. New grads welcome to apply and relocation assistance is offered. Please contact me for more details. Thank you! Melissa Owens Allied Search Partners ------------------------------ Message: 7 Date: Tue, 10 Mar 2015 20:43:38 +0000 From: "Hunter, Theresa" <[email protected]> Subject: [Histonet] Job opportunity To: "'[email protected]'" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hello everyone and happy National Histotechnologist Day! We are looking for a Histology Supervisor to lead a great team in Hershey Pa. If you are interested, please follow the link below. Thanks for your time, Theresa Career Website: http://www.pennstatehershey.org/web/humanresources/home/searchjobs The Penn State Milton S. Hershey Medical Center is committed to affirmative action, equal opportunity and the diversity of its workforce. Equal Opportunity Employer - Minorities/Women/Protected Veterans/Disabled. All individuals (including current employees) selected for a position will undergo a background check appropriate for the position's responsibilities Theresa Hunter, MLS(ASCP)cm Interim Assistant Manager AP Cytology/Decedent care/Histology Lead Pathologist Assistant Department of Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center 500 University Drive, Mail Code H160 Hershey, Pa. 17033 Phone (717) 531-0003 Ext. 287181 Fax (717)531-0831 [email protected]<mailto:[email protected]> ------------------------------ Message: 8 Date: Tue, 10 Mar 2015 20:50:27 +0000 (UTC) From: [email protected] Subject: [Histonet] TDP43, PTG poly To: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=utf-8 We stain TDP43 on both FFPE and frozen sections. The FFPE are done on the Bond, but we can't seem to get as good results with the frozen sections on the Bond. Our neuropathologist still prefers manually stained frozen sections for TDP43 over ones done on the Bond. We would really like to get it working reliably on the Bond. ??Does anyone have a protocol they would be willing to share for frozen sections on the Bond? Victor University of Washington Medical Center ------------------------------ Message: 9 Date: Wed, 11 Mar 2015 09:21:08 +0900 From: Rui TAHARA <[email protected]> Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification To: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="iso-2022-jp" Thank you for all the helpful suggestions about this topic. My sample has been in the fixative until it would be decalcified. Thank you again, rui > From: [email protected] > To: [email protected] > Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible > decalcification > Date: Tue, 10 Mar 2015 09:49:00 +0000 > > Leave it in formalin for as long as > possible..........................good luck > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Rui TAHARA > Sent: 04 March 2015 23:39 > To: [email protected]; [email protected] > Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible > decalcification > > Thank you for helpful suggestions. > I have further questions. > Yes, I have a bird head (probably 1 cm X 1 cm ) stored in 70 % ethanol. > But i have a similar size bird head fixed in 3.7% formalin for over night and > am actually processing the head to store in 70% ethanol since my lab is just > ordering the decalcifying solution. I need to decalcify this sample later. > But i am wondering if it is better to keep the sample in formalin for a week > or so till i get the decalcification solution or i should store it in 70 % > ethanol and then fix it for a few days again later? > I am afraid that longer fixative time would affect the sample somehow (e.g. > the sample become too rigid?) > > Thank you, > > rui > > > From: [email protected] > > To: [email protected] > > Date: Wed, 4 Mar 2015 16:25:28 -0700 > > Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible > > decalcification > > > > You wrote: > > > > > > > > I have an adult bird skull that fixed with formalin and then has been > > stored in 70% ethanol. > > > > I have seen the post that the sample stored in 70% ethanol can be > > walking back through to series of ethanol to water and can be > > decalcified if it needs to be. > > > > > > > > I am wondering if anybody has done this and there is any side effects > > from decalcification after going through dehydration and rehydration > > of a sample compared to a general straight forward protocol from > > decalcification to dehydration? > > > > ********************************************************************** > > ****** > > ********************************************************************** > > ****** > > ******************************** > > > > > > > > I have, in the past, when a weekend arrive, I interrupted acid bone > > decalcification by removing it from acid decalcifier, a quick water > > rinse and immersed into 70% alcohol before returning bone to fresh > > acid decalcifier the next working day. The bones always decalcified > > without problems but I am sure the decalcification took longer since > > partially decalcified bone had to rehydrate. I later learned more > > about dipolar (hope I said that correctly) alcohol slowing and/or stopping > > ionization of calcium > > and ceased using 70% alcohol to interrupt acid decalcification. I now use > > NBF to interrupt decalcification. Interestingly, I learned the alcohol > > technique from the AFIP bone pathology lab. > > > > > > > > Alcohol is put into Perenyi's nitric acid decalcifying solutions to slow > > down or control very rapid nitric acid decalcification. > > > > > > > > You did not say how big the bird skull was? I suggest immersing the skull > > back into NBF to let it totally rehydrate for several days (depending > > on skull size and if the brain is present). I suggest changing NBF if you > > rehydrate longer than a day. You don't need to go back through an alcohol > > gradient since many processing schedules have tissue samples going from NBF > > directly into 70%. If you leave residual alcohol in the bones, the acid > > decalcification could be slower and hopefully not retarded in any way. > > > > > > > > > > It certainly is worth a try. Good luck. > > > > > > > > Gayle M. Callis > > > > HTL/HT/MT(ASCP) > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > [email protected] > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Tue, 10 Mar 2015 16:39:35 +0000 (UTC) From: David Kemler <[email protected]> Subject: [Histonet] BIG day today! To: Histonet <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset=UTF-8 That's right, it's OUR day! NSH is doing their thing. Link here: http://www.nsh.org/content/histotechnology-professionals-day . Yours,Dave ------------------------------ Message: 11 Date: Tue, 10 Mar 2015 23:47:25 -0400 From: Mike <[email protected]> Subject: [Histonet] PA To: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license? Thanks Sent from my iPhone ------------------------------ Message: 12 Date: Tue, 10 Mar 2015 21:17:05 -0700 From: Jennifer MacDonald <[email protected]> Subject: Re: [Histonet] PA To: Mike <[email protected]> Cc: "[email protected]" <[email protected]>, [email protected] Message-ID: <of4c2a119d.ccc4f1ab-on88257e05.0015e2a0-88257e05.00177...@mtsac.edu> Content-Type: text/plain; charset="UTF-8" To be a PA you would need to have ASCP certification. There is one route to be eligible for certification. To be eligible for this examination category, an applicant must have a minimum of a baccalaureate degree from a regionally accredited college/university, AND successful completion of a NAACLS accredited Pathologists??? Assistant program within the last five years. Most of the NAACLS programs are Masters degrees. From: Mike <[email protected]> To: "[email protected]" <[email protected]> Date: 03/10/2015 08:49 PM Subject: [Histonet] PA Sent by: [email protected] Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license? Thanks Sent from my iPhone _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 11 Mar 2015 12:49:32 +0000 From: Nancy Schmitt <[email protected]> Subject: [Histonet] Diff Quik troubleshooting To: "'[email protected]'" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Good Morning- Random FNA case where the slides turn blue as they dry. We have tried taking them back through stains, destaining and restaining, etc. FNA smears are air-dried when we receive them on plain slides, unfixed. Any thoughts appreciated. Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 14 Date: Wed, 11 Mar 2015 08:52:05 -0400 From: Klaus Dern <[email protected]> Subject: [Histonet] THICK AND THIN SECTIONS ? To: histonet <[email protected]> Message-ID: <CAJhryisZAh9wS1MokgaT70LhoUh39_SqZYauW0Sovj8_sm_t=a...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 If you are using one of the following microtomes and are being told the advance mechanism is worn out. ( too much play between spindle and spindle nut ) You could be faced with purchasing a new Microtome. ( No parts availability ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. For Information, contact: Klaus Dern Phone: 706 635-8840 E-Mail: [email protected] ------------------------------ Message: 15 Date: Wed, 11 Mar 2015 15:42:45 +0000 From: "Mayer,Toysha N" <[email protected]> Subject: [Histonet] RE: Old slides To: "'[email protected]'" <[email protected]> Message-ID: <47e9b2c01dddd94881eacd2dc44ebc881d508...@d1pwpexmbx05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick <[email protected]> Subject: [Histonet] Old slides. To: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 [email protected]<mailto:[email protected]> ------------------------------ Message: 16 Date: Wed, 11 Mar 2015 12:53:13 -0300 From: Mariela Chertoff <[email protected]> Subject: [Histonet] 5-methylcytosine IHC in tissue To: [email protected] Message-ID: <CAC6nBUvyuxafo2tm-+A28+=hb506ydu3koq4kppvpyq7vul...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hi Has anybody experience with 5-metcyt staining in brain tissue? some advice about the HCl and Boric acid treatment? I use 50um free floating sections ferfused with PFA 4%. Thank you in advance for your help!! Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu??mica Biol??gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell??n II Piso 4 Ciudad Aut??noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email:[email protected] [email protected] <[email protected]> ------------------------------ Message: 17 Date: Wed, 11 Mar 2015 15:53:12 +0000 From: "Mayer,Toysha N" <[email protected]> Subject: [Histonet] RE: Masson Trichrome stain To: "'[email protected]'" <[email protected]> Message-ID: <47e9b2c01dddd94881eacd2dc44ebc881d508...@d1pwpexmbx05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver. As a rule of thumb, it is just easier to use plastic all the way around. The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal. That would be a stretch. As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further. This would affect the staining results. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan <[email protected]> Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf <[email protected]>, [email protected] Cc: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf <[email protected]> wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:[email protected]] > <[email protected]]> > Sent: Thursday, March 05, 2015 5:36 AM > To: [email protected] > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ ------------------------------ Message: 18 Date: Wed, 11 Mar 2015 16:04:35 +0000 From: "Marcum, Pamela A" <[email protected]> Subject: [Histonet] RE: Old slides To: "'Mayer,Toysha N'" <[email protected]>, "'[email protected]'" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" We have literally about one hundred slides to re-slip for the this reason. Are there any suggestions for large numbers of slides to be re-coverslipped as this method would be too time consuming. We have used only glass for about nine years or so and it is much better. The old ones are the problem when someone needs "THAT" slide only. Pam Marcum UAMS -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Mayer,Toysha N Sent: Wednesday, March 11, 2015 10:43 AM To: '[email protected]' Subject: [Histonet] RE: Old slides Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick <[email protected]> Subject: [Histonet] Old slides. To: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 [email protected]<mailto:[email protected]> _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 19 Date: Wed, 11 Mar 2015 16:52:22 +0000 From: "Solis, Bryan" <[email protected]> Subject: [Histonet] RE: Histonet Digest, Vol 136, Issue 12 To: "'[email protected]'" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hello, We received some liver tissue (Mouse) in 10% formalin (NFB). Then transferred to 70% ETOH. My question is that, Is it ok to transfer to 30% sucrose? So, frozen section can be perform. Please advise. Thanks, Bryan S. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Tuesday, March 10, 2015 10:06 AM To: [email protected] Subject: Histonet Digest, Vol 136, Issue 12 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Old slides. (Bernice Frederick) 2. RE: Old slides. (Jason McGough) 3. RE: Mushrooms for GMS fungus control (Morken, Timothy) 4. Re: FW: Masson's trichrome stain (John Kiernan) 5. RE: Old slides. (John Kiernan) 6. soft for microwriter (thermo scientific, Lamb, Shandon) (richard wild) 7. Re: Old slides. ([email protected]) 8. Re: Old slides. (Rene J Buesa) 9. RE: Old slides. (Gowan,Christie C) 10. IHC / Morphometry Technician wanted in Shenandoah Valley Virginia (Erin Sarricks) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick <[email protected]> Subject: [Histonet] Old slides. To: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 [email protected]<mailto:[email protected]> ------------------------------ Message: 2 Date: Mon, 9 Mar 2015 14:20:09 -0600 From: Jason McGough <[email protected]> Subject: RE: [Histonet] Old slides. To: Bernice Frederick <[email protected]>, [email protected] <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset=utf-8 Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 [email protected] <mailto:[email protected]> www.clinlab.com <http://www.clinlab.com> -----Original message----- > From:Bernice Frederick <[email protected] > <mailto:[email protected]> > > Sent: Monday, March 9, 2015 1:51 PM > To: [email protected] > <mailto:[email protected]> > Subject: [Histonet] Old slides. > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. > Sakura I would imagine. The issue here is that the coverslips have come up > from the slide and the tissue is adhered to the back of the coverslip. They > need to be recovered so they can be evaluated. What do you all recommend? We > use the CV5030 for coverslipping. I tried one with xylene and mounting media > but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > [email protected] <mailto:[email protected]> > <mailto:[email protected] > <mailto:[email protected]> > > > _______________________________________________ > Histonet mailing list > [email protected] > <mailto:[email protected]> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > ------------------------------ Message: 3 Date: Mon, 9 Mar 2015 22:46:08 +0000 From: "Morken, Timothy" <[email protected]> Subject: [Histonet] RE: Mushrooms for GMS fungus control To: "[email protected]" <[email protected]> Cc: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="utf-8" Try this article... Acta Cytol. 2003 Nov-Dec;47(6):1043-4. Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology. da Silva VD1. Author information Abstract OBJECTIVE: To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories. STUDY DESIGN: A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears. RESULTS: Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory. CONCLUSION: It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients. PMID: 14674076 [PubMed - indexed for MEDLINE] -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Sunday, March 08, 2015 4:29 PM To: Linda Prasad (SCHN) Cc: Jeffrey Robinson; [email protected] Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.?? Sounds like fun to me.?? I'm curious, with the emphasis now on quality control in labs??run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls??? A PI in research who??doesn't want??his paper rejected at peer review.?? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.?? The??dot-your-i's and cross-your-t's??FDA people who might or might not OK your drug in development.?? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA ----- Original Message ----- From: "Linda Prasad (SCHN)" <[email protected]> To: "Jeffrey Robinson" <[email protected]>, [email protected] Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: [email protected] | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???????Please consider the environment before printing this email. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: [email protected] Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? ??Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan <[email protected]> Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf <[email protected]>, [email protected] Cc: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf <[email protected]> wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:[email protected]] > <[email protected]]> > Sent: Thursday, March 05, 2015 5:36 AM > To: [email protected] > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 5 Date: Tue, 10 Mar 2015 00:40:02 -0500 From: John Kiernan <[email protected]> Subject: RE: [Histonet] Old slides. To: Jason McGough <[email protected]>, Bernice Frederick <[email protected]>, [email protected], [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=iso-8859-1 Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough <[email protected]> wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. > Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > [email protected] <mailto:[email protected] > <[email protected]>> > > www.clinlab.com <http://www.clinlab.com> > > ? > ? > -----Original message----- > > From:Bernice Frederick <[email protected] > > <mailto:[email protected] <[email protected]>> > > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: [email protected] > > <mailto:[email protected] > > <[email protected]>> > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. > > Sakura I would imagine. The issue here is that the coverslips have come up > > from the slide and the tissue is adhered to the back of the coverslip. They > > need to be recovered so they can be evaluated. What do you all recommend? > > We use the CV5030 for coverslipping. I tried one with xylene and mounting > > media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > [email protected] <mailto:[email protected] > > <[email protected]>> <mailto:[email protected] > > <[email protected]> <mailto:[email protected] > > <[email protected]>> > > > > > _______________________________________________ > > Histonet mailing list > > [email protected] > > <mailto:[email protected] > > <[email protected]>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 6 Date: Tue, 10 Mar 2015 10:05:11 +0100 From: richard wild <[email protected]> Subject: [Histonet] soft for microwriter (thermo scientific, Lamb, Shandon) To: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=utf-8; format=flowed Hi I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated. I would like to use serial interface rs232 and barcode scanners. Thanks for help. Richard ------------------------------ Message: 7 Date: Tue, 10 Mar 2015 11:51:25 +0000 From: [email protected] Subject: Re: [Histonet] Old slides. To: Bernice Frederick <[email protected]> Cc: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset=us-ascii Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's, Dublin, Ireland > On 9 Mar 2015, at 19:41, Bernice Frederick <[email protected]> > wrote: > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. > Sakura I would imagine. The issue here is that the coverslips have come up > from the slide and the tissue is adhered to the back of the coverslip. They > need to be recovered so they can be evaluated. What do you all recommend? We > use the CV5030 for coverslipping. I tried one with xylene and mounting media > but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > [email protected]<mailto:[email protected]> > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 10 Mar 2015 13:43:35 +0000 (UTC) From: Rene J Buesa <[email protected]> Subject: Re: [Histonet] Old slides. To: John Kiernan <[email protected]>, Jason McGough <[email protected]>, Bernice Frederick <[email protected]>, "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset=UTF-8 To John Probably the writer is referring to slides "mounted" with the Sakura film??"coverslip".I have done it many times and the FILM will??detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.Ren?? J.?? On Tuesday, March 10, 2015 1:40 AM, John Kiernan <[email protected]> wrote: Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough?? <[email protected]> wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. > Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > [email protected] <mailto:[email protected] <[email protected]>> > > www.clinlab.com <http://www.clinlab.com> > > ?? > ?? > -----Original message----- > > From:Bernice Frederick <[email protected] > > <mailto:[email protected] <[email protected]>> > > > Sent: Monday, March 9, 2015 1:51 PM > > To: [email protected] > > <mailto:[email protected] > > <[email protected]>> > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. > > Sakura I would imagine. The issue here is that the coverslips have come up > > from the slide and the tissue is adhered to the back of the coverslip. They > > need to be recovered so they can be evaluated. What do you all recommend? > > We use the CV5030 for coverslipping. I tried one with xylene and mounting > > media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > [email protected] <mailto:[email protected] > > <[email protected]>> <mailto:[email protected] > > <[email protected]> <mailto:[email protected] > > <[email protected]>> > > > > > _______________________________________________ > > Histonet mailing list > > [email protected] <mailto:[email protected] > > <[email protected]>> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 9 Mar 2015 20:01:10 +0000 From: "Gowan,Christie C" <[email protected]> Subject: [Histonet] RE: Old slides. To: "'Bernice Frederick'" <[email protected]>, "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: [email protected] Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 [email protected]<mailto:[email protected]> _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 10 Mar 2015 12:12:05 -0400 From: Erin Sarricks <[email protected]> Subject: [Histonet] IHC / Morphometry Technician wanted in Shenandoah Valley Virginia To: histonet <[email protected]> Message-ID: <CAPapK_1E+KJTXn9pLbWgLsVrd98Fdi1=ulpmhsbkssdb8nr...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Histology Laboratory located in the Shenandoah Valley of Virginia is looking to add to it's team. In this position, you will need a working knowledge of IHC theory and practical IHC experience. The best candidate for the position will oversee immunohistochemical staining as well as perform other histology functions including trimming of specimens, paraffin embedding, microtomy and microscopic QC of slides. Experience with morphometry is preferable. Desirable candidates will possess the following: - HT (ASCP) or QIHC registration preferred - 4 years of Histology experience - 1+ years of immunohistochemistry and/or immunofluorescence experience - Keeps abreast with company's current policies and immunohistochemistry technical updates and procedures - Must be able to work independently and in a team environment Full time employment benefits include subsidized medical and dental insurance, vacation, holiday pay, and 401k after 1 year of employment. Compensation is commensurate with experience. If you are interested in this position, please respond to this post with your resume and cover letter. ------------------------------ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 136, Issue 12 ***************************************** ------------------------------ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 136, Issue 13 ***************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
