> > I used 88% formic acid in Dwater to pretreatment of mouse tissue slides > before beta-Amyloid and Anti-Glial Fibrillary Acidic Protein (GFAP) > antibodies IHC. 10 minutes in formic acid with gental shaking before > blocking step.
You may also check this paper. Acta Neuropathol. <http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=C-terminal+alpha-synuclein+immunoreactivity+in+structures+other+than+Lewy+bodies+in+neurodegenerative+disorders.#> 2000 Mar;99(3):296-304. C-terminal alpha-synuclein immunoreactivity in structures other than Lewy bodies in neurodegenerativedisorders. Takeda A <http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Takeda%20A%5BAuthor%5D&cauthor=true&cauthor_uid=10663973> 1, Hashimoto M <http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Hashimoto%20M%5BAuthor%5D&cauthor=true&cauthor_uid=10663973> , Mallory M <http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Mallory%20M%5BAuthor%5D&cauthor=true&cauthor_uid=10663973> , Sundsumo M <http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Sundsumo%20M%5BAuthor%5D&cauthor=true&cauthor_uid=10663973> , Hansen L <http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Hansen%20L%5BAuthor%5D&cauthor=true&cauthor_uid=10663973> , Masliah E <http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Masliah%20E%5BAuthor%5D&cauthor=true&cauthor_uid=10663973> . Hope this wil help. Dorothy Hu HSDM > Message: 3 > Date: Fri, 10 Apr 2015 15:00:55 -0500 > From: Tyrone Genade <tgen...@gmail.com> > Subject: [Histonet] formic acid treatment and alpha-synuclein staining > To: histonet <histonet@lists.utsouthwestern.edu> > Message-ID: > < > caeyee3ma4xhp0_6brsqzqpujsohi15vdnzn+vkbrbb2pfws...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello, > > I'm do some research on alpha-synucleinopathies and how to stain them. I am > reading, from the Braak et al papers, that the slides need be treated for > 10 minutes with formic acid. The method is largely absent. I'm a biochemist > so I understand the formic acid is denaturing and unfolding the aggregates > and exposing the epitopes. Would this effect the ability to label other > epitopes such as plaques and tangles, tyrosine-hydroxylase, FOX2A etc..? > Does anyone have any experience in double-labeling sections for Lewy Bodies > and other proteins? > > In the same paper an alternative method of a 48 hr incubation on the slide > is suggested. Details of the staining buffer are absent but I'm hunting > them down and expect to find something like Triton X-100 in it which will > again unfold the aggregate (a little). > > Does anyone have a reliable protocol to share? I'm now several references > deep* from my original article and am still trying to find the paper that > describes the method. > > Thanks > -- > Tyrone Genade > > *Back in my Biochemistry days we played a game: Hunting for Bradford. > Essential, select a paper at random, see if it uses the Bradford Assay and > find out how many papers you had to read before arriving at the original > paper by Bradford. My then Prof held the record at some 30+ papers... > > Orange City, Iowa > tel: (+1) 712 230 4101 > http://tgenade.freeshell.org > > ******************************************************************************** > Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. > To find out how to receive this FREE gift visit http://www.alpha.org. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet