There are a couple of old techniques you might try.
1. Smear some Mayer's egg albumen on a slide as you would normally do,
i.e. very thinly, then hold it in a flame until it smokes. Allow to cool
and pick up the section. Allow to drain well.
2. Same as 1, but place the slide with the picked up tissue in a coplin
jar with a couple of mL concentrated formalin in the bottom for a hour
or so to fix the albumen.
3. Same as 1 and 2, but use 1% gelatin instead of Mayer's egg albumen.
4. If all else fails, stain free floating sections of about 15 microns
and pick up on a slide immediately before coverslipping. This was the
way fat stains were done years ago before the cryostat.
Bryan Llewellyn
Jo-Ann Bader, Ms. wrote:
We are having difficulty with a particulate set of very, very fatty mouse
livers. The normal livers from this set stay on the slides the fatty livers
fall off. We have used different types of charged slides and we have even
tried to drench the charged slides in Stay-On, dry them and then put the frozen
tissues on (despirate times call for despirate measures). No luck Does anyone
have any other ideas. Help Help
Jo-Ann Bader
Histology Coordinator
Goodman Cancer Research Center
1600 Pine Ave. W,
Room 312
Montreal Quebec, H3A 1A3
Email: jo-ann.ba...@mcgill.ca<mailto:jo-ann.ba...@mcgill.ca>
Office Tel: 514-398-5647
Lab: Tel: 514-398-8270
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