No ice forms in fixed, paraffin embedded tissue blocks at usual temperatures and length of chilling time and temperatures.
Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 20 Apr 2015 19:06:17 +0200 > From: j.benavi...@eae.csic.es > To: histonet@lists.utsouthwestern.edu > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > Hi there, > > I´m curious about the soaking thing. We have never done it in our lab. > Which is the purpose to do it? > > Than, after facing the blocks, we chill them in a cold plate so, if > wanting to do the soaking , when should we? I guess before placing them > on the cold plate, but that may cause a bit of ice formation? > > Thanks a lot for your help > > Julio > > > On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote: > > Rachel, > > First off, are you chilling and soaking the blocks after you face them? > > Do that and see if there is a difference. > > Don't try to get many sections to your ribbons. Shoot for a smaller ribbon > > (5-6) sections that are good. Cut slowly but consistently. > > What microtome are you using? Are you using disposable blades and are they > > sharp? Don't expect them to cut well if you use the same blade to face the > > blocks. If you aren't using disposables, get some! They will make your life > > easier. > > You might try to find a histotech at a local hospital lab who might be able > > to give you a hands-on lesson. > > Don't despair! We all sat down at our microtomes those first times and > > suffered trying to get perfect sections. It takes practice. You might make > > some blank blocks or blocks with tissue you can spare to practice your > > cutting techniques. I used to do this with my students and it really helped > > them. > > Good luck! > > > > Andi G. > > ________________________________________ > > From: histonet-boun...@lists.utsouthwestern.edu > > [histonet-boun...@lists.utsouthwestern.edu] on behalf of Rachel M Gonzalez > > [rac...@gbi-inc.com] > > Sent: Monday, April 20, 2015 9:07 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] prevent wrinkles when cutting > > > > Hi > > > > Thursday was the first time I ever used a microtome.... I move to a lab > > that does not have someone dedicated to cutting. I already miss her. > > > > I have no problems getting ribbons of 10-30 sections long but the pieces > > are half the size of the original block. I am guessing they are wrinkling. > > What am I doing wrong? > > > > Thanks > > Rachel > > Senior Scientist.... > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet