I remember reading that the preffered temperature was about 60 degrees Celsius.
I think that this was in the Dako education guides if I'm not mistaken. If that
is the case, the citation for the source is probably in that resource available
as pdf from their website.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: tony.henw...@health.nsw.gov.au
> To: wdesalvo....@outlook.com; preis...@mail.etsu.edu
> Date: Fri, 24 Apr 2015 09:43:59 +0000
> Subject: RE: [Histonet] IHC and oven temperature
> CC: histonet@lists.utsouthwestern.edu
>
> Hi temp drying shown to be a bad idea:
>
> Henwood, A., (2005) “Effect of Slide Drying at 80°C on Immunohistochemistry”
> J Histotechnol 28(1):45-46.
>
> Abstract
>
> Prolonged high temperature dry heating has been found to be deleterious to
> the immunohistochemical demonstration of several antigens in formalin-fixed,
> paraffin- embedded sections. Paraffin sections were dried at 80°C for 7 h and
> their immunoreactivity was compared with mirror sections dried for 1 h at
> 60°C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating
> whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to
> be quite resistant. It is recommended that coated slides (poly-L-lysine or
> aminopropyltriethoxysilane) and low-temperature drying (<60°C) be routinely
> used for irnmunohistochemistry.
>
> ________________________________________
> From: histonet-boun...@lists.utsouthwestern.edu
> [histonet-boun...@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO
> [wdesalvo....@outlook.com]
> Sent: Tuesday, 21 April 2015 1:56 AM
> To: Preiszner, Johanna
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] IHC and oven temperature
>
> Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are
> removing water trapped between slide and paraffin section. Antigen retrieval
> is an entirely different process. So not try to combine the two processes
>
> Sent from my iPhone
>
> > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna <preis...@mail.etsu.edu>
> > wrote:
> >
> > Hi Netters,
> >
> > is there something wrong with this logic:
> >
> > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C
> > oven."
> >
> > Of course I'll test it before I try it on real specimens, but maybe someone
> > else already knows the answer...
> >
> > Thanks!
> >
> > Hanna Preiszner
> > ETSU/QCOM
> >
> >
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