How long do you bake slides for before staining, at what temperature?  Does 
your stainer use agitation?

-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carolyn Nelson
Sent: Thursday, April 30, 2015 11:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi, I was hoping someone can help me with tissue falling off the slides. I have 
tried regular slides with and without adhesive in the water bath. Charged 
slides with and without adhesive in the water bath. I have not changed the type 
of slides I’m using. All the chemicals are fresh in the processor and the stain 
line, as well as the paraffin in the processor. It is the worst on needle bx ( 
prostate and breast ). I am SO frustrated, any help would be greatly 
appreciated! 


Carolyn






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