Huh? Take a solution more dilute than you want it and dilute it more?
Geoff On 5/1/2015 10:41 AM, Goins, Tresa wrote:
All that matters here is the final concentration of the reagent - it doesn't matter what stock you start with if you calculate the dilution. Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 = .0125 N final concentration. If using a 1 N stock solution, just add 10X the volume or 12.5 ml 1 N stock to 987.5 ml water. Hope this helps, Tresa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 30, 2015 1:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Question All, I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
