I intend to use a cryostat to cut 70 - 100 micron thick sections of human optic chiasmata.
Tissue is cryoprotected with 30% sucrose solution. My question relates to the freezing process per se. Would it be enough to place the tissue in a -4 freezer to harden and then transfer to the cryostat chamber at say -20 wait a while and cut? Or is it necessary to introduce an intermediary step for freezing? Thanks SS *Solomon Segal, M.D.* *Associate Professor of Anatomy in SurgeryCenter for Anatomical Science and Education (CASE)Department of SurgerySchool of MedicineSaint Louis University* *1402 South Grand Blvd.* *Schwitalla Hall - 3rd Floor - M310* *Saint Louis, MO, 63104office: 314 977 8023laboratory: 314 977 8080* *CASE: 314 977 8027FAX: 314 977 5127e-mail*: sseg...@slu.edu http://medschool.slu.edu/anatomy/ http://slu.academia.edu/SolomonSegal https://sites.google.com/a/slu.edu/segal-laboratory/ https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet