Formaldehyde 37% (commonly called 100% formalin) compared to 4% ( commonly known as 10% neutral buffered formalin)-in theory the 37% should fix quicker and better BUT anecdotally it is said that high concentrations of formaldehyde quickly form a "shell" in the tissue and will stop good penetration and fixation to the deeper tissues AND over the years it has been said anecdotally that 4% concentration is the quickest and most complete for all sample (mammal and plant) fixation and preservation-are these true. Please do discuss the methanol or buffers that is in the formaldehyde, or discuss paraformaldehyde (which is non polymerized formaldehyde with no methanol, in water).
Regards Peter Noyce PhD student. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet