Autopsy Program 13h 36m
Step Reagent Time Temp. Vac Stir Drip Time
1 Fixative 30 37 Cycle Medium 10
2 Fixative 30 37 Cycle Medium 10
3 Dehydrant 60 37 Cycle Medium 10
4 Dehydrant 60 37 Cycle Medium 10
5 Dehydrant 60 37 Cycle Medium 10
6 Dehydrant 60 37 Cycle Medium 10
7 Dehydrant 90 37 Cycle Medium 10
8 Dehydrant 90 37 Cycle Medium 10
9 Xylene 45 37 Cycle Medium 10
10 Xylene 60 37 Cycle Medium 10
11 Xylene 60 37 Cycle Medium 10
12 Wax 40 60 Cycle Medium 10
13 Wax 60 60 Cycle Medium 10
14 Wax 60 60 Cycle Medium 30
Works PERFECTLY every time!
Chris Simmons B.S., A.S., HTL(ASCP)
Supervisor, UPP Dermatopathology
412.864.3880 office
412.612.0881 cell
-----Original Message-----
From: Stoll, Kathryn [mailto:kst...@mcw.edu]
Sent: Monday, June 29, 2015 10:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Brain Tissue Processing
Hello,
I am looking for help with tissue processing of brain. The pieces are large,
they fill a super cassette. Does anyone have a protocol they could share.
Thanks in advance.
Kathryn Stoll
Supervisor Histology
Clinical and Translational Research Core Lab Medical College of Wisconsin
9200 W. Wisconsin Ave Room 1176
Milwaukee WI 53226
Phone: 414-805-1525
Fax: 414-805-1528
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
