Steve McClain writes: "In UPMC protocol They wrap all specimens, ink the tissue with eosin (erythrosine) at the grossing bench and cut two levels at 40 microns."
Do NOT use eosin for this purpose. It's highly fluorescent, and will interfere with any method (such as FISH) that relies on fluorescence. Safranin O works well for this purpose - placing the specimens on those blue pads after marking them. It's easily available - this is the red component of the Gram stain done by any microbiology service. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet