I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time.....
When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Jul 10, 2015, at 10:55 PM, John Kiernan <[email protected]> wrote: > > DPX is a polystyrene mounting medium. In principle you can make your own from > published recipes. In practice, everyone buys commercial resinous mounting > media. > > In the 1990s we had trouble similar to what you describe. The commercial DPX > was cloudy, and not because of alcohol in our xylene. The Canadian supplier > acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a > poly(methacrylate) plastic and is an excellent but expensive mounting medium. > Another poly(methacrylate) mountant called CytoSeal was less expensive and > also came in a squeeze-easy plastic bottle for delivery onto the slide or > coverslip. It's now my routine resious mountant. > > Good DPX returned to the market in the 2000s, but in old-fashioned bottles > and not easy to apply to slides or coverslips. > > John Kiernan > = = = >> On 09/07/15, Adam Boanas <[email protected]> wrote: >> Hello, >> >> We are having a problem that is developing into a big issue in our lab and I >> was wondering if anybody could shed any light on it. Our CV5000 coverslipper >> has recently started introducing microscopic air bubbles onto the slides >> during coverslipping. We have been told by our engineer that it is a >> consequence of the age and use of the motor and that sourcing another for an >> instrument that old (15yrs) will be v difficult. As such, we have been >> forced to manually coverslip using DPX and a pipette - manually applying the >> coverslips to the slide, thus mirroring the action of the coverslipper. This >> is fine at first and for the next few days the slides look great and very >> clean. However, after about day 4 -5 days post coverslipping, the slides >> develop an odd appearance down the microscope which looks like very fine >> `parched earth / crazy paving` all over the slide - including the section. >> The excess mountant around the edge of the coverslip also has a very faint, >> cloudy appearance wh! >> en this occurs. This of course renders the slide un-useable. Does anyone >> have a clue what this might be down to / how we can stop it? >> We are struggling for ideas with this one! - this occurs with fresh DPX also. >> >> Many thanks >> Adam >> >> Adam Boanas >> Senior Research Associate >> Epistem Ltd >> 48 Grafton Street >> Manchester, M13 9XX >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
