Question: When we stain and H&E on frozen we fix in 95% prior to staining. As I never in 30 years have done a Masson's Trichrome on a frozen section I ask : do I need to fix and then mordant as usual or go straight to the Bouin's and continue as normal? Do I need to mordant at all? Tissue is rabbit liver. BErnice
Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet