Mouse knee joints: done lots of decalcified FFPWS for assessment of articular cartilage degeneration models. See Histonet images for a Tol blue image. Decal in 10 % EDTA for 3 days on a rocker at RT. Sure....5days if you are worried. No difference in Immuno-reactivity, imho. If you want to use buffered Formic acid, use Formic acid; sodium formate. Use of citric acid with Formic acid does not make a buffer. It's just mixing two relatively mild acids..... However, I am sure that Prof Kiernan can further enlighten us.
Respectfully, Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
