Hello, I will be performing a number of immunos within the next couple of months, and have been wondering what the best way to do so might be with no tissue damage during the process. Does anyone know whether using NetWell inserts for immunos to transfer tissue affects morphology? The tissue will be placed into the insert, and submersed in solution within a well plate to be incubated on a shaker. The problem is that contact with the NetWell insert could damage the tissue, not only with the rocking, but with transfer from one solution to another.
I am under the impression that with TUNEL staining, is often difficult to differentiate whether the damage in cells caused by handling/extraction, or from the treatment itself. While not impossible, I will not have the time to develop or perform such a procedure. If anyone has any knowledge or insight, it would be greatly appreciated. Thank you for your time, Emily Dewar Laboratory Technician _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet