Histonetters! Our go-to stain is SafO for any bone/cartilage, but we always have the option to use toluidine or alcian blue.
Just going out on a limb, but has anyone stained tissues with SafO/Fast Green, without using any alcohols after the SafO step? These samples have some issues when put into alcohol and if we wash the SafO in water, instead of alcohol, the stain isn't correct. What is it about the alcohol step after SafO that makes it work so well? And why does water cause such a problem? Next week, I will try a lower alcohol percentage after the SafO step to see how that works. Thanks, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet