Taking a poll:  How many of your labs recycle formalin, alcohol and xylene?  

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From: histonet-requ...@lists.utsouthwestern.edu 
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Sent: Thursday, July 09, 2015 11:00 AM
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Subject: Histonet Digest, Vol 140, Issue 9

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Today's Topics:

   1. 2 Open Positions in Little Rock Arkansas (Marcum, Pamela A)
   2. Gross Room QC sheet (Histology Technician)
   3. Medica ELGA DV 25 (Histology Technician)
   4. Roche B-gal Staining Kit (Coffey, Anna (NIH/NCI) [C])


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Message: 1
Date: Thu, 9 Jul 2015 15:15:47 +0000
From: "Marcum, Pamela A" <pamar...@uams.edu>
To: Histonet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] 2 Open Positions in Little Rock Arkansas
Message-ID: <dc5416a8415544ceb9cca4c9f981c...@mail13m2n2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"

WE currently have two open positions for either registered HT or HTL.  Registry 
must be current and some experience.  We are a heavily computerized Histology 
Laboratory and will require the persons applying to be comfortable with 
computers.  We are a routine Histology Laboratory at the University of Arkansas 
for Medical Sciences, in Little Rock.  This is a beautiful area in Central 
Arkansas with lots of things to do both indoors and especially outdoors.   If 
you are interested please contact me with your resume as soon as possible.


Please no recruiters, we are not allowed to use you,  I am sorry.

Thank You,

Pam Marcum

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Message: 2
Date: Thu, 9 Jul 2015 16:20:09 +0000 (UTC)
From: Histology Technician <histology81...@att.net>
To: Histonet <histonet@lists.utsouthwestern.edu>,
        "histonet-requ...@lists.utsouthwestern.edu"
        <histonet-requ...@lists.utsouthwestern.edu>
Subject: [Histonet] Gross Room QC sheet
Message-ID:
        <1290933179.1997863.1436458809680.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

 Does anyone have a Gross Room QC sheet you'd like to share?? Thanks so much!

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Message: 3
Date: Thu, 9 Jul 2015 16:22:47 +0000 (UTC)
From: Histology Technician <histology81...@att.net>
To: Histonet <histonet@lists.utsouthwestern.edu>,
        "histonet-requ...@lists.utsouthwestern.edu"
        <histonet-requ...@lists.utsouthwestern.edu>
Subject: [Histonet] Medica ELGA DV 25
Message-ID:
        <377965148.2007366.1436458967925.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

 Does anyone use the DI water system called Medica by Eqova?? We just recently 
got 2 in our lab and was wondering if anyone had an SOP you'd like to share?? 
Can you tell my what daily QC needs to be done with it?? Water tested?? Thanks!

------------------------------

Message: 4
Date: Thu, 9 Jul 2015 16:57:14 +0000
From: "Coffey, Anna (NIH/NCI) [C]" <anna.cof...@nih.gov>
To: "histonet@lists.utsouthwestern.edu"
        <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Roche B-gal Staining Kit
Message-ID: <5c3e10119a1b824fbe92b08279f74a9102598...@msgb10.nih.gov>
Content-Type: text/plain; charset="us-ascii"

Hello,

I have been asked to do a B-gal enzyme stain using a Roche kit on frozen mouse 
sections. Another histologist recommended post-fixing in a 
glutaraldehyde-formaldehyde solution although this isn't mentioned in the kit 
instructions. This has me worried that there may be other things missing from 
the kit instructions that could improve the results. Has anyone else had any 
experience using this kit and, if so, did you find that other alterations to 
the protocol were required? Also wondering about potential counterstains that 
might work with this one. I'd appreciate any pointers if you have them!

Thanks!
Anna

Anna Coffey, MS, HTL(ASCP)CM
Histotechnologist
Center for Advanced Preclinical Research Frederick National Laboratory for 
Cancer Research Leidos Biomedical Research, Inc.
Bld 539, 224
Frederick, Maryland 21702
anna.cof...@nih.gov
301-846-1730



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End of Histonet Digest, Vol 140, Issue 9
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