Hello, my simple mind is thinking: can you scan before extracting the brain? or, can you extract the brain under water?
Michael Baker, M.D. (CCHMC Pathology) > Message: 11 > Date: Wed, 26 Aug 2015 16:10:21 +0100 > From: Ekaterina Vinnik > Subject: rodent brain: how to avoid air bubbles in the ventricles after > perfusion? > > Dear all, > I'm new to this list - and was really happy to find it. Does anybody have > any advice on how to do rat brain extraction in such a way that the air > would not get into the ventricles? We are doing MRI on the ex vivo rat > brains, and the air which gets in causes nasty artifacts. I am looking for > consistent way to avoid the bubbles, cause . > The way we are extracting the brain now is to isolate the head, peel the > skull from the top and from the sides, crush the nasal bone, expose the > bulbs, remove dura, cut the nerves, lift the brain.. I was never concerned > with this problem until I started doing MRI - and here it turns out to be > really important. > > I was wondering if I should just leave the dura in place during extraction, > for example, would that work? > > Thank you in advance! > Katya > > -- > Ekaterina Vinnik, MD, PhD > Behavioural neuroscience lab, > Champalimaud Neuroscience Programme, > Lisbon, > Portugal > > +351918951392 > lulswin...@gmail.com ---- Michael Baker, M.D. CCHMC Pathology T (513) 636-4261 F (513) 636-3924 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
