Hi, Peroxidase can really be a pain. If you look in the archives though (or ask her really nice) Gayle Callis submitted a recipe for a glucose oxidase for peroxidase quenching that does not include hydrogen peroxide. If you aren't really a fan of making these things up I would bet dimes to doughnuts that the recipe is *really* similar to the product from Biocare Medical called PeroxAbolish. Here's a link... http://biocare.net/product/peroxabolish/ I have used it and it did work, but I don't really use it regularly so can't really compare it well. Incidentally if I am wrong in my assumptions about the similarity of this to the glucose oxidase, I trust someone here (even from the company itself) will gently correct me.
Cheers, Amos On Sat, Sep 12, 2015 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Message: 5 > Date: Fri, 11 Sep 2015 20:33:45 +0000 > From: "Lewis, Patrick" <patrick.le...@seattlechildrens.org> > To: " (Histonet@lists.utsouthwestern.edu)" > <Histonet@lists.utsouthwestern.edu> > Subject: [Histonet] More on H202 issues > Message-ID: > > <3903be18914f4440834f0e620415ffca3cba0...@ppwexd01d.childrens.sea.kids> > > Content-Type: text/plain; charset="us-ascii" > > > Hi Everyone > > Thanks for your responses. > > I am looking at cell surface markers, > Sorry I should have said. > > Based on what I found out below: > > Methanol is out, even though I agree that Methanol does enhance the effect > of H202 blocking. > (I suppose I could try it to see how much/if any epitope loss there is in > relation to H202 quenching, At least It would help identifying false > positives that are actually H202 artifacts.) > > Also it looks like increasing the concentration of H202 is out. > > As to when though, > > It looks like with cell surface markers I should block after the primary, > or even after the 2ndary? > Can I do that successfully with a HRP labeled 2ndary? > > thoughts? > > Patrick. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet